Knockout gata4 gene and establish ment of a zebrafish model of congenital heart disease by TALEN / 中国比较医学杂志

Objective To establish a gata4 gene knockout zebrafish model of congenital heart disease, and construct transcription activator-like effector nuclease ( TALEN) vectors targeting gata4 gene.Method We construct TALEN vectors targeting zabrafish gata4 gene using unit assembly method and the in vitro-transcribed TALEN mRNAs were microinjected into one-cell stage zebrafish embryos.The efficiency of TALEN was identified by injected embryos, and mutations of zebrafish were screened and confirmed the different types through PCR and enzyme digestion.Results We successfully constructed correct targeting vectors by enzyme digestion and sequencing, and the gene knockout efficiency was 35.18%.We screened the mutant zebrafish and confirmed different types of gata4 gene mutations.Conclusions A gata4 knockout zebrafish model is successfully established, it can provide a good animal model for further research of congenital heart diseases.

Research of zebrafish quality standardization / 中国实验动物学报

At present, zebrafish has played an increasingly important role in models for human development and diseases and several areas of life sciences.As a newly laboratory animal resource, standardization research has become the technical bottleneck to be solved and an inevitable trend.In this review, we summarized the research history and character-istics of zebrafish and the status of quality standardization.We also discussed the main problem facing by the standardiza-tion research of zebrafish as a newly laboratory animal.We hope that the data can provide useful reference for the develop-ment of zebrafish quality standardization research.

Establishment of the mouse B cell mitogenic-and co-mitogenic monoclonal antibodies / 中国免疫学杂志

Sibirian hamster was immunized with mouse B myeloma cell(A20)lysate,and the im-munized spleen cells were fused with mouse myeloma cell line X63Ag8653.Four mono-clonal antibodies were thus obtained.While two(4B4 and 3C11)antibodies were shown to have strong direct mitogenic ac-tivity on mouse spleen B cells,all 4 antibodies could have synergistic effect in coope-rate with suboptimal doses of LPS to activate B cells.The mitogenic activity is ac-cessory cell independent.Four different surface molecules,as judged by both immuno-precipitating and binding assay,were compared.And the usefulness of these antibodiesin studying B cell activation and their functions were discussed.

Idiopathic Scoliosis Impair Patients' Pulmonary Ventilation / 第二军医大学学报

Pulmonary ventilation function of 22 patients with idiopathic scoliosis was assessed. The results showed that there were significantly lower in Vital Capacity, Vita! Capacity % in predicted, Inspiratory Capacity, Forced Vital Capacity, Forced Expiratory Volume in Is, Maximal Voluntary Ventilation, and Maximal Voluntary Ventilation % in predicted of patients than those of age and sex matched normal controls. And there was no any statistical difference of Tidal Volume, Forced Expiratory Volume in Is % in predicted, Maximal Middle Flow, and Maximal Middle Flow % in predicted between two groups. Furthermore, the reduction of Vital Capacity % in patients with idiopathic scoliosis was correlated with the Cobb Angle, Wedge Index and rotation degree of the top vertebral body and not with the scoliosis direction and the patients' age.

STUDIES ON THE ROLE OF SUPPRESSOR T CELL IN RESISTANCE TO EXPERIMENTAL AUTOIMMUNE ENCEPHALITOMYELITIS / 中国免疫学杂志

In order to test whether suppressor T cells are the responsible cell com-ponent in the induced resistance of Lewis rat against reinduction of Exp-erimental Autoimmune Encephalomyelitis(EAE)or not,attempts have beenmade to enrich a T cell population,which were functionally antagonistic tothe encephalitogenic activity of the EAE-inducing autoaggressive T cells,Lewis rats were passively induced for acute EaE by intravenous injectionof encephalitogenic,Myelin Basic Protein(MBP)-specific T line cell(S1cell). Nylon-wool-enriched spleen T cells recovered from animals passivelyinduced EAE,were cultured in vitro with irradiated encephalitogenic Tcells(S1)in the absence of antigen.The selective growth of T cell popula-tion with the phenotype of CD8 were found in the cultures.Functional ch-aracteristics of this cell population were studied both in vivo and in vitro.Thus,the S1-stimulated cells(Anti-S1 cells)are able to suppress both theantigen-and Con A-induced T cell proliferation of the antigenspecificT cell series.Studies on the in vivo activities of the Anti-Si cells couldhave shown that,pretreatment or posttreatment of the normal Lewis reci-pients with isolated Anti-Si cells are able to abolish or prevent thedisease-inducing activity of autoaggressive T cells.

Studies on the Heterogeneity of Antigen-Presenting Cells. Establishment of T cell Hybridomas specific for Different Antigen-Presenting Cells / 中国免疫学杂志

A series of alloreactive T hybridomas were obtained using fusion between BW147 and C3H/HeN T cell lymphoblasts, which were prepared by stimulating With lpsactivatedBalb/c B cells Six of the total 89 hybridomas tested have been shown to respond selectively to LPS-activated B cells, but not spleen adherent cells, macrophage tumor line and Ia-transfected fibroblasts. The results indicated: 1) Different Antigen-Presenting Cells could selectively stimulate the allogeneic responses of distinctive clonal T cells; 2) The specific stimulating effects may either due to the structural differences among the Ir-genes of different cell types, or the same gene produce(s), which may form different molecular complexes on the membrane surfaces of different cell types, lead to their functional specificities.